Abstract: the application of combined nitrogen, especially nitrate, to soybean plants is known to strongly inhibit nodule formation, growth and nitrogen fixation in the present study, we measured the effects of supplying 5 mm nitrate on the growth of nodules, primary root, and lateral roots under light at 28 °c or dark at 18 °c conditions. 31 effect of nitrate in soybeans growth in order to know the effect of nitrate on soybeans growth, a set of plants inoculated with b japonicum usda110, napa and nosz denitrification mutants were cultivated with mineral solution containing 0, 1, 2 or 4 mm nitrate respectively shoot and root dry weight, nodule number and nodule fresh and dry weight were measured. The application of combined nitrogen, especially nitrate, to soybean plants is known to strongly inhibit nodule formation, growth and nitrogen fixation.
Effect of the symbiotic nitrogen fixation on soybean soybeans planted in low nitrogen soil without rhizobium bacteria +l: soybeans planted in low nitrogen soil with rhizobium bacteria -h: soybeans planted in high nitrogen soil without rhizobium bacteria +h: soybeans planted in high nitrogen soil with rhizobium bacteria can be nodulated. Effect of nitrate on nodulation and nitrogen fixation of soybean 335 a: photograph of soybean nodules formed in the roots of a plant cultivated by hydroponics. Soybeans (glycine max) and the cowpea group (which includes the acacia mangium and acacia senegal) are infected by the bradyrhizobium bacteria by supplying the host with nitrogen , an individual rhizobium can enhance host photosynthesis, presumably increasing the rhizobium’s own access to photosynthate.
The process of nodulations affects the amount of available nitrate or ammonia, and level of concentrations in the soil which results mostly in depress nodulations another the n2 fixation in active, growing, and nodulated legumes rate are suppressed by no3 ions. Generally, soil with high level of nitrogen (applied or residual) affects the nodulations and n2 fixation in effect the increased in nitrogen application will increase plant growth and grain yield of soybeans, thus, root and shoot size of soybeans also increases refernces: adjei, mb, kh quesenberry and cg chambliss 1999. The inhibition of nodule growth in soybeans by nitrate is also complex effect of nitrate supply to the culture solution on the growth of the primary root under light and dark conditions sodium nitrate was added to n free solution to prepare the culture solution with 5 mm nitrate photographs of the nodulated roots in the glass.
The effect of nitrate on n2 fixation and the assimilation of fixed n2 in legume nodules was investigated by supplying nitrate to well established soybean ( glycine max l merr cv bragg)- rhizobium japonicum (strain 3i1b110) symbioses. Effect of nitrate on nodule and root growth of soybean (glycine max ( l) merr) the effect of supplying 5 mm nitrate on lateral r oot growth was different from that on primary.
Analyses of the saps and stem extracts for ureides (allantoin plus allantoic acid), α-amino-n, and nitrate, and of dry matter for n and 15n, indicated a positive effect of nitrate supply on concentrations of nitrate in saps and extracts and a negative effect on ureides and on the proportion of plant n derived from nitrogen fixation.